Figure 6B

A. Schematic representation of the experiment methodology for this figure. B. Supernatants from infected MRC-5 cells expressing either vector or MyD88 at an MOI of 0.05, with TB40/E-mCh, were collected every three days for 15 days and filtered through 0.2 mm disc filter. The filtered supernatants from each day were then added to another monolayer of MRC-5 infected with TB40/E-mCh at an MOI of 0.05 every three days by removing the equivalent volume of the filtered supernatant from the well (therefore 1:1 ratio with the media in the well). Cells were monitored for virus spread by imaging every three days. C. Supernatants from infected MRC-5 cells expressing either vector or MyD88 were collected at 7 dpi and filtered through a 0.2 mm disc filter. They were then either untreated or denatured by boiling at 95° C before storage. The un-boiled and boiled supernatants were added on another monolayer of MRC-5 infected with TB40/E-mCh at MOI of 0.05 every three days by removing the equivalent volume of the filtered supernatant from the well (therefore 1:1 ratio with the media in the well). The spread of the virus was monitored by imaging every three days. D. A monolayer of MRC-5 infected with TB40/E-mCh at an MOI of 0.05 was treated with mock (UT), IFN-β (50ng/ml), or IL1-β (100ng/ml). The spread of the virus was monitored every three days by confocal imaging up to day 15. The spread of infection was quantified as the area of fluorescence using the NIS element software. Results are shown as mean ± SD. * p<0.05.