Integrated Proteomic and Genomic Analysis of Gastric Cancer Patient Tissues

V-erb-b2
erythroblastic leukemia viral oncogene homologue 2, known
as ERBB2, is an important oncogene in the development of certain cancers.
It can form a heterodimer with other epidermal growth factor receptor
family members and activate kinase-mediated downstream signaling pathways.
ERBB2 gene is located on chromosome 17 and is amplified in a subset
of cancers, such as breast, gastric, and colon cancer. Of particular
interest to the Chromosome-Centric Human Proteome Project (C-HPP)
initiative is the amplification mechanism that typically results in
overexpression of a set of genes adjacent to ERBB2, which provides
evidence of a linkage between gene location and expression. In this
report we studied patient samples from ERBB2-positive together with
adjacent control nontumor tissues. In addition, non-ERBB2-expressing
patient samples were selected as comparison to study the effect of
expression of this oncogene. We detected 196 proteins in ERBB2-positive
patient tumor samples that had minimal overlap (29 proteins) with
the non-ERBB2 tumor samples. Interaction and pathway analysis identified
extracellular signal regulated kinase (ERK) cascade and actin polymerization
and actinmyosin assembly contraction as pathways of importance in
ERBB2+ and ERBB2– gastric cancer samples, respectively. The
raw data files are deposited at ProteomeXchange (identifier: PXD002674)
as well as GPMDB.