Transcriptome analysis after the lamprey larvae infected by three pathogenic microorganisms

Based on the large number of deaths of lamprey larvae infected by pathogens in the culture process, the infection response of lamprey larvae to different pathogens was studied. Thus ，high-throughput sequencing is firstly used to analyze the lamprey larvae infected by Aeromonas hydrophila, Vibro Splendidus, and Saprolegnia parasitica. Based on the transcriptome of lamprey larvae infected by three aquatic pathogens, classification results of COG and GO show that the genes classified into signal transduction mechanism accounted for the largest proportion, and 2316 differentially expressed genes were identified and screened out. KEGG pathway analysis of differentially expressed genes showed that lampreys larvae was mainly affected their metabolism and oxidative phosphorylation pathways after infection by two gram-negative bacteria, Vibro Splendidus and Aeromonas hydrophila. As a fungus, Saprolegnia parasitica mainly affected ribosome synthesis in lamprey larvae. The results were validated by detecting the expression levels of 13 DEGs at 24 h after pathogenic bacteria treatment using Q-PCR. The results provides valuable information for further research into the development of immunity and the innate immune response against bacterial invasion during the artificial propagation of lamprey larvae.