Supplementary materials - Gut bacterial metabolites and biomarkers of leaky gut in atopic dermatitis

Supplemental Table 1. The list of laboratory parameters investigated in the study. LC-MS – liquid chromatography-mass spectrometry; ELISA – enzyme-linked immunosorbent assay. Supplemental Figure 1. A hypothesis substantiating the concept of the gut-skin axis in atopic dermatitis (left hand side) and a graphical depiction of the study design (right hand side). Alterations in food- and microbiome-derived metabolites may trigger gut barrier impairment. This could lead to systemic inflammation, atopic dermatitis flare-ups and increased risk of food sensitization. The hypothesis was verified in a cross-sectional study of 50 patients with active atopic dermatitis and 25 controls. Bacterial metabolites, biomarkers of gut barrier damage, cytokines and food-specific IgE were analyzed and correlated to clinical characteristics of the study group. AD – atopic dermatitis; EASI – Eczema Area and Severity Index; ELISA – enzyme-linked immunosorbent assay; LC-MS – liquid chromatography-mass spectrometry; SCORAD – SCORing Atopic Dermatitis. Supplemental figure, table, and methods