Figure 2_qPCR.xlsx

<strong>Figure 2:</strong> Quantitative PCR of CALCR mRNA extracted from U87-MG cells subjected over time (BL, baseline; 2 hr, 5 hr, 8 hr &amp; 24 hr) to stress with depleted FBS, no FBS and treatment with 1 μM staurosporine. The qPCR panels were generated for <em>CALCR</em>Total (37), <em>CALCR</em>b (D-F) and compared to <em>CALCLR</em> mRNA (CT receptor-like Receptor, G-I). There were no statistical differences for <em>CALCR</em> transcripts across the time periods for any treatments, but for <em>CALCLR</em> mRNA there was a significant one way downward trend over 24 hr for dFBS and no FBS (panel G, *p = 0.0002; panel H, *p = 0.0008: one way ANOVA with repeated measures, linear trend). Data are mean +/- SD.