Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Toe1 konckout Transcriptomes in differentiated C17.2 cells

We extracted RNA from NC and KO-Toe1 differentiated C17.2 cells for high-throughput transcriptomic sequencing. In differentiated C17.2 cells, Toe1 primarily affected biological functions including peptidase activity, chemotactic factors, the ECM, and the TNF signaling pathway. Overall design: mRNA profiles of NC and KO-Toe1 were generated by deep sequencing using Illumina GAIIx.