Effect of PT2385 treatment during cytotrophoblast differentiation under hypoxia

To investigate the therapeutic effect of HIF-2 inhibitor in placental dysfunction, we used primary cytotrophoblasts isolated from human placentas at term and cultured them under 2.5% O2 for 4 days. We either treated the cells with PT2385 at 10 µmol/L or vehicle and then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different placentas. Comparative gene expression profiling analysis of RNA-seq data for treated and untreated cytotrophoblasts.