Figure 5C - Peptides eluted from MHC class I molecules in Hela+IFNy and Hela-TAPBPR KO+IFNy

Peptide-MHC class I complexes were isolated by affinity chromatography using W6/32 from IFN-γ treated HeLa and HeLa-TAPBPR KO cells. Eluted peptides were analysed using LC-MS/MS. Peptides were assigned as HLA-A*68:02 and HLA-B*15:03 binders based on their peptide motifs using the online programme NetMHC.本研究采用亲和层析法，使用W6/32试剂，从经干扰素-γ（Interferon-γ, IFN-γ）处理的海拉（HeLa）细胞及海拉-TAPBPR基因敲除（Knockout, KO）细胞中分离肽-主要组织相容性复合体I类复合物（peptide-MHC class I complexes）。随后通过液相色谱-串联质谱法（Liquid Chromatography-Tandem Mass Spectrometry, LC-MS/MS）对洗脱获得的肽段进行分析。基于肽段的基序特征，借助在线程序NetMHC，将上述肽段归类为人类白细胞抗原A*68:02（HLA-A*68:02）与人类白细胞抗原B*15:03（HLA-B*15:03）的结合肽。