The complete genome sequence of the thermophilic bacterium Laceyella sacchari FBKL4.010 reveals the basis for tetramethylpyrazine biosynthesis in Moutai‐flavor Daqu

The genus Laceyella consists of a thermophilic filamentous bacteria. The pure isolate of Laceyella sacchari FBKL4.010 was isolated from Moutai‐flavor Daqu, Guizhou Province, China. In this study, the whole genome was sequenced and analyzed. The complete genome consists of one 3,374,379‐bp circular chromosome with 3,145 coding sequences (CDSs), seven clustered regularly interspaced short palindromic repeat (CRISPR) regions of 12 CRISPRs. Moreover, we identified that the genome contains genes encoding key enzymes such as proteases, peptidases, and acetolactate synthase (ALS) of the tetramethylpyrazine metabolic pathway. Metabolic pathways relevant to tetramethylpyrazine synthesis were also reconstructed based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) PATHWAY database. Annotation and syntenic analyses using antiSMASH 4.0 also revealed the presence of two gene clusters in this strain that differ from known tetramethylpyrazine synthesis clusters, with one encoding amino acid dehy...莱西拉菌属（Laceyella）为一类嗜热丝状细菌。本研究的实验菌株为从中国贵州省茅台香型大曲中分离得到的糖生莱西拉菌（Laceyella sacchari）纯分离株FBKL4.010。本研究对该菌株的全基因组进行了测序与分析。其完整基因组包含一条长度为3,374,379 bp的环状染色体，共编码3145个编码序列（CDSs），并含有7个成簇规律间隔短回文重复序列（clustered regularly interspaced short palindromic repeat，CRISPR）区域，共计12个CRISPR位点。此外，本研究鉴定到该基因组携带有编码四甲基吡嗪代谢通路关键酶的基因，包括蛋白酶、肽酶以及乙酰乳酸合酶（ALS）。基于京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路数据库，本研究还重构了与四甲基吡嗪合成相关的代谢通路。通过antiSMASH 4.0进行基因注释与共线性分析后发现，该菌株存在两个不同于已知四甲基吡嗪合成基因簇的基因簇，其中一个编码氨基酸脱氢...