Mass spectrometry raw data for "Proteomics reveals substantial differences between in vitro matured abattoir-derived and in vivo matured oocytes in cattle"

In vitro production (IVP) of bovine embryos still has its limitations such as low blastocyst rate and lower embryo quality, resulting in lower pregnancy rates following the transfer of IVP embryos compared to in vivo produced embryos. Given these differences in developmental competence, RNA sequencing and microarray technology have been applied to describe the differences in transcriptional activity between in vitro and in vivo produced embryos. All but one of these studies solely utilized oocytes obtained from slaughterhouse material for the in vitro production of embryos, thereby introducing the possibility, that differences between IVP and in vivo embryos are in part attributable to differing sources of oocytes. The aim of the present study was therefore to compare the proteome of oocytes retrieved from slaughterhouse material, with and without a period of in vitro maturation and in vivo matured oocytes obtained from donor cattle following superovulation. For each group the protein pattern of four biological replicates containing ten oocytes each were analyzed via SWATHTM-MS.