The in vitro influence of orexin B on the porcine myometrium on days 15 to 16 of gestation.

Orexin A (OXA) and orexin B (OXB) are hypothalamic-derived peptides that participate in the regulation of energy metabolism, food intake and reproductive function by influencing the hypothalamic-pituitary-ovarian axis. Orexins are also produced in the endometrium, myometrium and placenta, which suggests that they could act as a link between energy metabolism and the reproductive system. The aim of this study was to explore the influence of orexin B on global gene expression in the porcine myometrium during early gestation, on days 15 to 16 of pregnancy (implantation period). We analized samples obtained from 4 indyviduals (n=4). Obtained from the uterine horns tissue explants were divided for two groups: controls [without treatment] and orexin B treated groups. The concentration of orexin B in the culture medium was 10 nM. In vitro tissue cultures were cultivated with treatments for 24 hours.