Over expression of mRNA for multiple genes including insulin in the PLN of NOD is associated with Islet Autoimmunity

The aim of this study is to identify genes implicated in the early steps of the autoimmune process, prior to inflammation in type 1 diabetes. Early Insulin AutoAntibodies (E-IAA) have been used as subphenotypic marker to select individual animals as type 1 diabetes prone and to compare gene expression patterns with insulin autoantibody negative NOD. Variation of gene expression patterns in the pancreatic lymph nodes (PLN) issued from E-IAA positive and negative mice have been analyzed by DNA microarrays PLN were used from 5 weeks old NOD mice positive for Insulin Autoantibodies and compared with animals negative for Insulin AutoAntibodies本研究旨在鉴定1型糖尿病炎症发生前，自身免疫过程早期阶段所涉及的基因。早期胰岛素自身抗体（Early Insulin AutoAntibodies，E-IAA）已被用作亚表型标记物，用于筛选易患1型糖尿病的个体动物，并将其与胰岛素自身抗体阴性的非肥胖糖尿病（Non-Obese Diabetic, NOD）小鼠进行基因表达谱对比。研究通过DNA微阵列（DNA microarrays）技术分析了胰岛素自身抗体阳性与阴性小鼠的胰腺淋巴结（pancreatic lymph nodes, PLN）中的基因表达谱差异；本研究选用5周龄胰岛素自身抗体阳性的NOD小鼠的胰腺淋巴结，并与胰岛素自身抗体阴性的NOD小鼠进行对照比较。