Transcriptomic profiling of microglia and astrocytes throughout aging

Purpose:The goal of this study are to understand the role of microglia and astrocytes in both physiological and pathological conditions. Methods: Microglia and astrocytes were isolated from 5 time points WT/APP-PS1 mice in triplicate. mRNA were generated by SMART seq2 using Illumina Novaseq platform. Conclusions: Using DESeq2 to sequence polyA-selected mRNAs from microglia and astrocytes isolated from whole brain. We mapped >85% of reads for all of our sample. We found good reproducibility between the replicates We performed RNA-seq in microglia and astrocytes freshly isolated from Wild-type(WT)/APP-PS1(AD) mice brains across the lifespan to analyze the longitudinal genes expression profile.研究目的：本研究旨在探究小胶质细胞（microglia）与星形胶质细胞（astrocytes）在生理及病理状态下的作用机制。研究方法：从5个时间点的野生型（Wild-type, WT）/APP-PS1小鼠体内分离小胶质细胞与星形胶质细胞，每组设置3次生物学重复。采用SMART seq2技术构建测序文库，并依托Illumina NovaSeq测序平台完成mRNA测序。研究结果与结论：本研究采用DESeq2对从全脑分离的小胶质细胞与星形胶质细胞的聚腺苷酸选择性mRNA测序数据进行分析。所有样本的测序reads比对率均超过85%，生物学重复间具有良好的重复性。本研究对整个生命过程中新鲜分离的野生型（WT）/APP-PS1（阿尔茨海默病模型，AD）小鼠全脑小胶质细胞与星形胶质细胞进行RNA测序（RNA-seq），以分析其纵向基因表达谱。