SMRT-seq on CRISPR/Cas9-edited beta-globin gene in a sickle HUDEP2 cell model with fluorescent reporter (GFP/BFP)

By repectively linking GFP and BFP reporter downstream HBB gene in each allele, our cell model enables monitoring of allele-specific beta-globin expression in live cells through GFP and BFP gene tagging. The SMRT-seq was applied to quantify the rates, size, allele, and relative position of large deletions in relation to the Cas9 cut site.