Aztreonam-induced filamentation in Pseudomonas aeruginosa

Objectives: The proliferation of metallo-beta-lactamase-producing Pseudomonas aeruginosa represents a significant public health threat. P. aeruginosa can undergo significant phenotypic changes that can drastically impair antibiotic efficacy. This study’s objectives were (1) to quantify the time course of killing of VIM-2-producing P. aeruginosa in response to aztreonam-based therapies, and (2) to document the capacity of P. aeruginosa to undergo morphological transformations that facilitate persistence.
Methods: A well-characterized, clinical VIM-2-producing P. aeruginosa was studied in the Hollow Fiber Infection Model (HFIM) over 9 days (7 days of active antibiotic therapy, 2 days treatment withdrawal) at a 107.5 CFU/mL starting inoculum. HFIM treatment arms included: growth control, aztreonam, ceftazidime/avibactam, aztreonam/‌ceftazidime/‌avibactam, polymyxin B, and aztreonam/‌ceftazidime/‌avibactam/‌polymyxin B. In addition, real-time imaging studies were conducted under static cond..., , , # Aztreonam-induced filamentation in Pseudomonas aeruginosa

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Author: Nicholas M. Smith, PharmD, PhD
Contact: [[e-mail]](mailto:[e-mail])
Last Update: 2023-07-20

These videos were generated through timelapse microscopy of a clinical VIM-2-producing P. aeruginosa under selective pressure by aztreonam and after drug removal.

## Description of the data file and structure

2020_20_31_782_Exponential Growth_MHB.wmv
2020_20_31_782_Filamentation_AZT_67.wmv
2020_20_31_782_Reversion_6hr.wmv
2020_20_31_782_Reversion_18hr.wmv
2020_20_31_782_Reversion_24hr.wmv
2020_20_31_782_Reversion_48hr.wmv

2020_20_31_782_Exponential Growth_MHB.wmv
-This timelapse video is of P. aeruginosa in exponential growing phase without drug pressure

2020_20_31_782_Filamentation_AZT_67.wmv
-This timelapse video is of P. aeruginosa in expoenntial growing phase under drug pressure (aztreonam 66mg/L, static conditions), which shows bacterial filamentation

2020_20_31_782_Reversion_6hr.wmv

* Th...