Next Generation Sequencing Analysis of Wild Type and SRSF2-/- D11 mice liver Transcriptomes

We generated the liver specific SRSF2 -/- mice, and collected their liver at 11 day after birth and controls. Then, we extracted RNAs and performed next generation sequencing. By comparing sequencing data from WT and SRSF2 -/- samples, we profiled the alternative splicing events and gene expression regulated by SRSF2 during mouse liver development process. D11 liver mRNA profiles of wild type (WT) and liver specific SRSF2-/- mice were generated by deep sequencing, using Illumina HiSeq2000.本研究构建了肝脏特异性SRSF2纯合敲除小鼠模型，并于小鼠出生后第11天采集其肝脏组织及对照样本。随后提取RNA并开展下一代测序（next generation sequencing）实验。通过对比野生型（wild type，WT）与SRSF2纯合敲除样本的测序数据，我们解析了小鼠肝脏发育过程中受SRSF2调控的可变剪接事件与基因表达谱。本研究采用Illumina HiSeq2000平台完成深度测序（deep sequencing），获取了野生型（WT）及肝脏特异性SRSF2纯合敲除小鼠出生后第11天的肝脏mRNA表达谱。