Lappula duplicicarpa var. brevispinula C.J.Wang (Boraginaceae) is a synonym of L. macrantha based on morphological and molecular data

Upon examining the type specimens of Lappula duplicicarpa var. duplicicarpa and L. duplicicarpa var. brevispinula, significant morphological differences were observed between these two taxa, indicating that the classification of L. duplicicarpa var. brevispinula as a variety may not be warranted. In this study, we conducted a comprehensive morphological comparison of L. duplicicarpa var. duplicicarpa, L. duplicicarpa var. brevispinula, and L. macrantha, alongside reconstructing a phylogenetic tree based on complete chloroplast genome data. The analyses suggest that L. duplicicarpa var. brevispinula and L. duplicicarpa var. duplicicarpa differ significantly in the morphology of the calyx, corolla, and eremocarps, as well as in the micro-morphological traits of the eremocarp. Statistically significant discontinuities were also observed in the sizes of the corolla and eremocarps. Notably, the morphological characteristics of L. duplicicarpa var. brevispinula fall within the range of variat..., , , # Molecular and morphological data of Lappula duplicicarpa var. brevispinula [https://doi.org/10.5061/dryad.j9kd51cnz](https://doi.org/10.5061/dryad.j9kd51cnz) ## Description of the data and file structure Molecular and morphological data of *Lappula duplicicarpa* var. brevispinula. Total genomic DNA was extracted from silica-gel dried leaves of L. duplicicarpa var. *duplicicarpa*, *L. duplicicarpa* var. *brevispinula*, and *L. macrantha* using a modified CTAB method (Li et al. 2013). High-quality genomic DNA was sent to Novogene Biotechnology in Tianjin for library construction and sequencing. Libraries were sequenced on the NovaSeq 6000 platform, generating paired-end reads of 2×150 bp, with approximately 10 Gb of raw data per sample. Plastome assembly was performed using GetOrganelle version 1.7.5 (Jin et al. 2020) with default parameters, and annotation was carried out using the online tool CpGAVAS2 (Shi et al. 2019), with *Lappula lasiocarpa* (Accession: NC_077516) as the refer...通过对双果鹤虱（*Lappula duplicicarpa*）原变种（var. duplicicarpa）与短刺双果鹤虱（*L. duplicicarpa* var. brevispinula）的模式标本进行检视，发现两类群间存在显著形态差异，表明将短刺双果鹤虱划归为变种的分类处理或并不恰当。本研究对双果鹤虱原变种、短刺双果鹤虱以及大花鹤虱（*L. macrantha*）开展全面形态学比较，并基于完整叶绿体基因组（chloroplast genome）数据构建系统发育树。分析结果显示，短刺双果鹤虱与双果鹤虱原变种在花萼、花冠及小坚果（eremocarp）形态，以及小坚果的微观形态特征上均存在显著差异；二者在花冠与小坚果的尺寸上也呈现统计学上显著的间断分布。值得注意的是，短刺双果鹤虱的形态特征变异范围……# 短刺双果鹤虱的分子与形态学数据 https://doi.org/10.5061/dryad.j9kd51cnz ## 数据与文件结构说明 短刺双果鹤虱的分子与形态学数据。 本研究采用改良十六烷基三甲基溴化铵（CTAB）法（Li等，2013），从双果鹤虱原变种、短刺双果鹤虱及大花鹤虱的硅胶干燥叶片中提取总基因组DNA。将高质量基因组DNA送往天津诺禾致源生物技术有限公司进行文库构建与测序。文库在NovaSeq 6000测序平台上完成测序，产出2×150 bp的双端读段（paired-end reads），每个样本的原始数据量约为10 Gb。使用GetOrganelle v1.7.5（Jin等，2020）的默认参数进行质体基因组组装，并以毛花鹤虱（*Lappula lasiocarpa*，登录号：NC_077516）为参考序列，通过在线工具CpGAVAS2（Shi等，2019）完成基因组注释……