Variations in the presence and distribution of methyl-CpG in genomic DNA observed across the tree of life are both biologically interesting and useful in modern genomic studies. Here we illustrate and discuss the use of human methyl-CpG binding domai

Variations in the presence and distribution of methyl-CpG in genomic DNA observed across the tree of life are both biologically interesting and useful in modern genomic studies. Here we illustrate and discuss the use of human methyl-CpG binding domain (H-MBD) as a tool for the fractionation of angiosperm total genomic DNA into eukaryotic nuclear (methyl-CpG rich) vs organellar and prokaryotic (methyl-CpG poor) enriched fractions for genomic and metagenomic sequencing projects.