Genome-wide maps of ZBTB38 in human cells

ZBTB38 is a transcription factor with affinity for methylated sequences in vitro. Here, we address ZBTB38 binding selectivity in vivo by mapping 3232 ZBTB38 binding sites throughout the human genome. These target sites are over-represented in Alu-repeats, and in the CpG island shores of highly expressed genes, hundreds of base pairs upstream of the transcription start site. In addition, the ZBTB38 target sites are highly similar to E2F4-binding motifs, but they are methylated, not bound by E2F4, and the corresponding genes are not cell-cycle regulated. Overall design: ChIP-seq of ZBTB38 in HeLa-S3 cells expressing a tagged ZBTB38