Underlying data for "A deep mutational scanning platform to characterize the fitness landscape of anti-CRISPR proteins"

Underlying data for the manuscript "A deep mutational scanning platform to characterize the fitness landscape  of anti-CRISPR proteins".

Contains FACS and NGS read data for the deep mutational scanning experiments carried out on the anti-CRISPR proteins AcrIIA4 and AcrIIA5.

The included tar.gz archive has the following directory structure:

fractions_a4/rep_1, rep_2 and rep_3: contains .fastq.gz files for 3 biological replicates of the 4 fractions sequenced for deep mutational scanning analysis of AcrIIA4

fractions_a5/rep_1, rep_2 and rep_3: contains .fastq.gz files for 3 biological replicates of the 4 fractions sequenced for deep mutational scanning analysis of AcrIIA5

library_a4/: contains .fastq.gz files for 2 replicates of the initial AcrIIA4 library

library_a5/: contains .fastq.gz files for 2 replicates of the inital AcrIIA5 library

count_matrices/: contains CSV-format count matrices with the number of reads per single-amino-acid variant for both sequenced fractions and the initial library

facs/ : contains .fcs files for the set of measured benchmark single mutants of AcrIIA4 and AcrIIA5, which were used for model training.