Fig. S5 Duplication mechanisms and conserved motif of UGT33 family in H. melpomene.

MCScanX (max gaps = 50) was used to identify the segmental and tandem duplications of detoxification genes in H. melpomene. This annotated tree was predicted by the collinear and tandem relationships of the output from ‘family tree plotter of MCScanX’. The characters T and S on the nodes mean tandem and segmental duplications, respectively. All the UGT33 family members in Fig. 4 were used to create sequence logo of signature region (http://weblogo.berkeley.edu/logo.cgi).采用MCScanX（最大间隙参数设为50），鉴定红带袖蝶（Heliconius melpomene）体内解毒基因的片段重复与串联重复事件。该带注释的进化树，系基于MCScanX家族树绘图器的输出结果中所包含的共线性与串联重复关系预测所得。进化树节点上标注的T与S，分别代表串联重复与片段重复事件。本研究选取图4中的全部UGT33家族成员，用于构建特征区域的序列logo图（http://weblogo.berkeley.edu/logo.cgi）。