The effect of PI3K inhibitors and cytochalasin D on net cholesterol accumulation in wild-type macrophages.

Wild-type bone marrow-derived macrophages were pretreated 1 h with either drug vehicle, 50 µM LY294002, 100 nm wortmannin, or 4 µg/ml cytochalasin D. Macrophages then were incubated with 1 mg/ml LDL and inhibitor for 6 h. Incubations were performed in serum-free medium containing 50 ng/ml M-CSF. The percent inhibition of net cholesterol accumulation compares macrophages treated with LDL alone and LDL with inhibitor after basal cholesterol values were subtracted from each. ** = pp