Sexually dimorphic and clock-gene specific effects of artificial light at night on Drosophila behavioral rhythms

Light pollution is a major anthropogenic environmental change and a significant threat to ecosystems. Among other detrimental effects on physiology, light at night (LAN) disrupts circadian rhythms in a wide range of species. However, the underlying neuronal and genetic mechanisms remain poorly understood. Here, we show that the loss of the circadian clock gene period (per) exacerbates the LAN-induced shift toward nocturnal behaviour, with a more pronounced effect on males. In contrast, the loss of cycle has no such effect on males or females; cyc null mutants are nocturnal under standard light‒dark cycles, and their activity and sleep profiles are minimally or not affected by LAN exposure. CRISPR-Cas9 knockout of per in most clock neurons resembles the null mutant phenotype. Our results show that mutations in components of the positive and negative limbs of the circadian clock result in distinct responses to nocturnal light and highlight the role of genetic factors in modulating behavio..., , # Dataset README **Article Title**: *Sexually dimorphic and clock-gene specific effects of artificial light at night on *Drosophila* behavioral rhythms* (2025) Pranevicius A., Biondi G., Iyer A.R., Seiffe A., Depino A.M., and Fernandez M.P. **Journal**: *Proceedings of the Royal Society B* **Dataset DOI**: [10.5061/dryad.wwpzgmswz](https://doi.org/10.5061/dryad.wwpzgmswz) **Contact**: Maria de la Paz Fernandez ([[e-mail]](mailto:[e-mail])) --- ## Description of the Data and File Structure * All data files are in `.csv` format. * Each file includes descriptors for sex, environmental condition (and genotype, when applicable). All experiments were conducted under a 12:12 light-dark cycle with either complete darkness at night (LD) or dim white light (ALAN). Zeitgeber time 0 (ZT12) indicates lights-on transition and Zeitgeber time 12 (ZT12) indicates lights-off transition (under LD) or start of the dim light phase (ALAN). Circadian time 0 (CT0) indicates the start of the su...,光污染是一类重要的人为环境改变，对生态系统构成显著威胁。除对生理机能产生诸多有害影响外，夜间光照（Light at Night, LAN）会干扰广泛物种类群的昼夜节律。然而，其背后的神经元与遗传机制仍不甚明晰。本研究表明，昼夜节律钟基因period（per）的缺失会加剧夜间光照诱导的夜行行为偏移，且该效应在雄性个体中更为显著。与之相反，cycle基因的缺失对雌雄个体均无此类影响；cyc无效突变体在标准明暗周期下仍保持夜行性，其活动与睡眠特征几乎不受夜间光照暴露的影响。在大多数节律神经元中通过CRISPR-Cas9敲除per基因，可重现该无效突变体的表型。本研究结果显示，昼夜节律钟正负调控通路组分的突变，会使个体对夜间光照产生迥异的应答，并凸显了遗传因素在调控行为中的作用（原文此处未完成） # 数据集说明文档 **论文标题**：*《夜间人工光照对果蝇*Drosophila*行为节律的性别二态性与节律基因特异性效应》（2025）* **作者**：Pranevicius A.、Biondi G.、Iyer A.R.、Seiffe A.、Depino A.M. 与 Fernandez M.P. **期刊**：《英国皇家学会学报B辑》（*Proceedings of the Royal Society B*） **数据集DOI**：[10.5061/dryad.wwpzgmswz](https://doi.org/10.5061/dryad.wwpzgmswz) **联系人**：Maria de la Paz Fernandez（邮箱：[e-mail]） --- ## 数据与文件结构说明 * 所有数据文件均采用`.csv`格式。 * 每个文件均包含性别、环境条件（适用时还包含基因型）相关描述字段。所有实验均在12:12明暗周期下开展，夜间分别设置为完全黑暗（LD组）或弱白光（ALAN组）。授时时间0（ZT0）代表灯光开启时刻，授时时间12（ZT12）在LD组中代表灯光关闭时刻，在ALAN组中则代表弱光阶段的起始时刻。昼夜时间0（CT0）代表活动的起始时刻（原文此处未完成）