Analysis of differential expressed genes between ad- and abaxial rosette leaves

To analysis of differential expressed genes between ad- and abaxial rosette leaves, we sequenced the adaxial side and abaxial side of leaves from twenty 4-week-old A. thaliana [L.] Heynh. ecotype Columbia plants. With the use of double-sided tape and a blade, the tissue on the abaxial and adaxial sides was collected separately. Total RNA was extracted from the two sides of leaves using the TransZol reagent.