Gene expression profile at single cell level of DKO and TKO prostate tumor

To explore how Pygo2 expression in tumor cells regulate the TME, we used single cell RNA sequencing (scRNA-seq) to analyze the immune cell composition and activity change in tumor area Single cell suspension were obtained from nDKOLuc or nTKOLuc tumor at 10 week or 17 week post tamoxifen inducing, singlce cells were labeled with hashtag and sent combined to load for 10xGenomics scRNA-seq