Effect of anti-osteoporotic treatments on circulating and bone microRNA patterns in osteopenic ZDF ratsEffect of anti-osteoporotic treatments on circulating and bone microRNA patterns in osteopenic ZDF rats

We studied microRNA expression levels from serum and ulna bone in an in vivo model of type II diabetes (ZDF rats) by investigating different anti-osteoporotic treatments (anti-sclerostin, PTH and insulin). Serum and ulna bone RNA were extracted and subject to small RNA sequencing on an Illumina NextSeq550 SR75 High using 75bp, single end reads. After mapping against Rnor.6.0 provided by Ensembl and miRBase v22.1 differential expression analysis was undertaken with edgeRv3.32 using a quasi-likelihood negative binomial generalized log-linear model. We identified a panel of altered small non-coding RNAs. Overall design: Examination of small non-coding RNAs in serum and ulna bone samples of ZDF rats under different anti-osteoporostic treatments using pairwise comparisons.