Ne estimates for the systemic colonization of pea leaves by PSbMV between 22 and 61 days post inoculation (dpi).

Estimates were obtained by two different methods and separately for two inocula corresponding to two different initial ratios of PSbMV variants. 95% confidence intervals estimated by bootstrapping among plants are indicated in brackets. The variable fi,j is the relative frequency of virus variant 1 in plant i and leaf j (j in {1,2,3} for date 1 and j in {4,5,6} for date 2) (see Fig. S1 for details on its estimation).aFor inocula 1 and 2, i∈[1–10] and i∈[11–19], respectively.bFor inocula 1 and 2, i∈[20–29] and i∈[30–39], respectively.cThe variance and FST methods assume an increase of the variance of viral frequencies (respectively of the FST statistics of viral populations) with time. “NA” (not available), indicates situations where these assumptions were not satisfied and, consequently, where genetic drift was negligible (Ne tends to infinity).