Bile acid receptor FXR promotes intestinal epithelial ferroptosis and subsequent ILC3 dysfunction in neonatal necrotizing enterocolitis [IEC bulk RNA-seq]

Necrotizing enterocolitis (NEC) is a prevalent pediatric emergency characterized by the sudden onset of intestinal ischemia and necrosis resulting from hyperinflammation.The interactions between the bile acid receptor FXR with gut microbiota and mucosal immunity in early postnatal life, and its exact role in the pathogenesis of NEC, are unknown.Here we analyze the role of FXR in IECs under NEC. IECs (EpCAM+CD45-) from Fxrfl/fl naïve, FxrΔIEC naïve, Fxrfl/fl NEC and FxrΔIEC NEC pups were sorted via BD FACS. Sorted cells were subjeted to bulk RNA-seq