Transcriptome landscape of the adrenal gland and superior cervical ganglion from hypertension-induced left ventricular hypertrophy rat model

Hypertension-induced left ventricular hypertrophy (LVH) is a cardiac structural remodeling and dysfunction resulting from chronic hypertension and is an independent risk factor for cardiovascular morbidity and mortality. Studies have implicated the involvement of the adrenal gland (AG) and superior cervical ganglion (SCG) in hypertension regulation. However, the molecular mechanisms of AG and SCG during hypertension-induced LVH remain unclear. In this study, we investigated the transcriptome characteristics of the left ventricle, AG, and SCG in 24-week-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats using transcriptome sequencing (RNA-seq) technology. We identified 671 differentially expressed genes (DEGs) in the left ventricle, 1596 DEGs in the AG, and 343 DEGs in the SCG between SHR and WKY rats. Functional analysis revealed that these DEGs were involved in pathways related to immune inflammation, metabolic responses, and synaptic transmission. Further analysis demonstrated the presence of crosstalk among DEGs across these three organs, including 63 overlapping DEGs among these three organs, of which 59 DEGs exhibited consistent changes. In summary, we elucidates the transcriptome features of AG and SCG during hypertension-induced LVH in SHR, highlighting the complex molecular interactions across multiple organs.高血压诱导性左心室肥厚（LVH）是慢性高血压引发的心脏结构重塑与功能障碍，亦是心血管疾病发病率与死亡率的独立危险因素。已有研究证实肾上腺（AG）与颈上神经节（SCG）参与高血压的调控过程，但高血压诱导性左心室肥厚进程中肾上腺与颈上神经节的分子机制仍不明确。本研究采用转录组测序（RNA-seq）技术，对24周龄自发性高血压大鼠（SHR）与Wistar-Kyoto（WKY）大鼠的左心室、肾上腺及颈上神经节的转录组特征进行分析。结果显示，相较于WKY大鼠，SHR大鼠的左心室中鉴定出671个差异表达基因（DEGs），肾上腺中鉴定出1596个差异表达基因，颈上神经节中鉴定出343个差异表达基因。功能分析表明，这些差异表达基因参与免疫炎症、代谢应答及突触传递相关通路。进一步分析发现，这三个器官的差异表达基因间存在串扰关系：三者共有63个重叠差异表达基因，其中59个基因的表达变化趋势一致。综上，本研究阐明了自发性高血压大鼠高血压诱导性左心室肥厚进程中肾上腺与颈上神经节的转录组特征，揭示了多器官间复杂的分子互作关系。