Rac1 controls both the secretory function of the mammary gland and its remodelling for successive gestations

To study the effects of Rac1 gene deletion on development and function of the mammary gland. The microarray was conducted from RNA isolated from mouse mammary glands harvested from Involution day 2 mice. Cre-mediated specific Rac1 gene deletion in luminal mammary epithelial cells was achieved by crossing Rac1fx/fxRosaYFP mice with WAPiCreTg/ mice to produce Rac1fx/fxYFP ;WAPiCreTg/ (Rac1-/-) mice. Rac1fx/fxYFP mice that lacked the Cre gene were used as wild type (WT) controls. The RNA was isolated from the inguinal gland 4 for each mouse.本数据集旨在探究Rac1基因敲除对乳腺发育与功能的影响。实验以乳腺退化期第2天小鼠的乳腺组织分离得到的RNA为样本，开展微阵列（microarray）检测。通过将Rac1fx/fxRosaYFP小鼠与WAPiCreTg/小鼠杂交，成功构建可在乳腺腔上皮细胞中实现Cre介导的特异性Rac1基因敲除的Rac1fx/fxYFP;WAPiCreTg/（即Rac1敲除型，Rac1-/-）小鼠。将不携带Cre基因的Rac1fx/fxYFP小鼠作为野生型（wild type，WT）对照。所有实验所用RNA均从每只小鼠的第4腹股沟乳腺中分离得到。