Identification of interactors of Sth1 in Candida albicans

Regulation of gene expression programs is crucial for the survival of microbial pathogens in host environments and for their ability to cause disease. Here we investigated the epigenetic regulator RSC (Remodels the Structure of Chromatin) in the most prevalent human fungal pathogen Candida albicans. To precisely determine the composition of the CaRSC complex, we immunopurified Myc-tagged Sth1, the core catalytic subunit of the CaRSC complex encoded by C3_02490C and identified its physical interactors using high-resolution tandem mass spectrometry (LC-MS/MS). Wild-type cells expressing untagged Sth1 served as negative control, and all immunoprecipitation experiments were analyzed in biological triplicates. No antibody controls for immunopurification were also analysed.