Madagascar Hissing Cockroach hemolymph PO2, PCO2, and gas exchange parameters

AbstractThis data set contains in vivo measurements of hemolymph PO2 and PCO2 made on Madagascar hissing cockroaches (Gromphadorhina portentosa) using implanted micro fiber optic sensors (optodes). In addition, rate of CO2 release, ventilation frequency, and durations of the tri-phasic discontinuous gas exchange cycle associated with these measurements are also recorded. All measurements were performed while the cockroaches were breathing either normoxic and normocapnic air, hypoxic air (10 kPa PO2), or hypercapnic air (2 kPa PCO2) at 22 ºC., MethodsThese data were collected and analyzed according to the materials and methods in the associated JEB article. Briefly, Madagascar hissing cockroaches (Gromphadorhina portentosa) were secured in a respirometry chamber with PCO2 and PO2 optodes implanted in their hemocoel. A reflection sensor beneath their abdomen recorded abdominal pumping movements. Cockroaches were exposed to normoxic normocapnia, hypoxic normocapnia, and normoxic hypercapnia. Roaches were exposed to these gas mixtures while intact and breathing continuously, and then decapitated which induced discontinuous gas exchange cycles (DGCs), before being exposed to the same gas mixtures as before. During these exposures, hemolymph PO2, PCO2, VCO2, and abdominal movements were recorded continuously. The summary data extracted form these data traces are presented here., Usage notesEach treatment assumed 6 cockroaches were used. Empty cells indicate that either equipment failure resulted in a parameter (e.g. hemolymph PCO2) not being recorded from that individual insect, or the insect did not display the breathing pattern required to record a certain parameter (e.g., the cockroach began breathing discontinuously, preventing the recording of continuous gas exchange parameters).摘要 本数据集包含采用植入式微光纤光极传感器（micro fiber optic sensors，optodes）对马岛发声蟑螂（Madagascar hissing cockroaches，Gromphadorhina portentosa）开展的体内血淋巴（hemolymph）氧分压（PO2）与二氧化碳分压（PCO2）测量数据。此外，数据集还同步记录了与上述测量相关的二氧化碳释放速率、换气频率以及三相不连续气体交换循环（tri-phasic discontinuous gas exchange cycle）的持续时长。所有测量均在22℃环境下进行，实验蟑螂分别呼吸常氧常碳空气、低氧空气（10 kPa PO2）或高碳酸空气（2 kPa PCO2）。 方法 本数据集按照关联的《实验生物学杂志》（Journal of Experimental Biology，JEB）论文中的材料与方法完成采集与分析。简言之，实验所用马岛发声蟑螂（Gromphadorhina portentosa）被固定于呼吸代谢舱（respirometry chamber）内，其血腔（hemocoel）中植入了PCO2与PO2光极传感器；腹部下方的反射式传感器用于记录蟑螂的腹部搏动运动。实验蟑螂先后暴露于常氧常碳、低氧常碳及常氧高碳酸三种气体环境。首先在蟑螂完整且持续呼吸的状态下使其接触上述气体混合物，随后对其实施断头处理以诱导不连续气体交换循环（discontinuous gas exchange cycles，DGCs），之后再次使其接触此前的气体混合物。在暴露阶段，研究人员持续记录血淋巴PO2、PCO2、二氧化碳排出率（VCO2）以及腹部运动数据。本数据集呈现的即为从上述连续数据轨迹中提取的汇总数据。 使用说明 每组实验处理均使用6只蟑螂。表格中的空值代表两种情形：一是因设备故障未能采集到对应个体的某一参数（如血淋巴PCO2）；二是该蟑螂未呈现出记录该参数所需的呼吸模式，例如蟑螂开始进行不连续呼吸，导致无法记录连续气体交换相关参数。