RNA-Sequencing from cluster architecture (peduncle and pedicels) of grape

Cluster compactness is an undesirable trait in Sistan Yaghooti grape. The RNA sequencing technique was applied for transcriptomic analysis to study the cluster development process in Sistan Yaghooti grape. The transcriptional complexity of this grape was studied in three stages of cluster development by RNA-Seq.The first stage, cluster formation time (Sample-1): when the approximate size of clusters were about 5 cm and the flowers had not been opened.The second stage, berry formation time (Sample-2): two weeks after the first sampling, when about 70% of the berries had been formed and the approximate size of clusters were about 15 centimeters.The third stage, final size of cluster time (Sample-3): two weeks after the second sampling, when the berries had reached about 70% of their final size.Berries were removed to extract the RNA from cluster architecture (peduncle and pedicels).Gibberellin was applied in concentration of 40 mg/lit for three samples (T1, T2 and T3). Gibberellin concentrations were determined according to previous researches [9,12-14]. To unify environmental conditions, the control samples (C1, C2, and C3) were sprayed by distilled water, simultaneously.