Data of manuscript Adaptation of Saccharomyces species to high-iron conditions

Abstract: Iron is an indispensable element that participates as an essential cofactor in multiple biological processes. However, when present in excess, iron can engage in redox reactions that generaten reactive oxygen species that damage cells at multiple levels. In this report, we have characterized the response of budding yeast species from the Saccharomyces genus to elevated environmental iron concentrations. We have observed that S. cerevisiae strains are more resistant to high-iron concentra tions than Saccharomyces non-cerevisiae species. Liquid growth assays showed that species evolu tively closer to S. cerevisiae, such as S. paradoxus, S. jurei, S. mikatae, and S. arboricola were more resistant to high iron levels than the more distant species S. eubayanus and S. uvarum. Remarkably, S. kudriavzevii strains were especially iron sensitive. Growth assays in solid media suggested that S. cerevisiae and S. paradoxus were more resistant to the oxidative stress caused by elevated iron concentrations. When comparing iron accumulation and sensitivity, different patterns were observed. As previously described for S. cerevisiae, S. uvarum and particular strains of S. kudriavzevii and S. paradoxus became more sensitive to iron while accumulating more intracellular iron levels. However, no remarkable changes in intracellular iron accumulation were observed for the rest of the species. Consistent with the activation of iron detoxification systems, an increased expression of the vacuolar iron transporter CCC1 was observed for iron-sensitive strains with high endogenous iron levels. These results indicate that different mechanisms of response to elevated iron concentrations exit in the different species of the genus Saccharomyces.