Transcriptional profiling of cDC2 subsets in IFNAR∆CD11c mice treated with allergens

Conventional type 2 Dendritic Cells (cDC2) in the skin are cell subsets primarily responsible for the priming of T-helper type 2 (TH2) responses in the draining LN. The local inflammatory milieu has been demonstrated to condition cDC2 to promote such responses. To characterise the cDC2 response to the cytokine IFN-I, we performed bulk RNAseq on sorted cDC2 subsets from IFNAR∆CD11c and IFNARWT mice following treatment with an allergen. IFNAR∆CD11c mice were treated with CTO-labelled inactivated N. brasiliensis larvae intradermally, and 48h later, cDC2 subsets carrying the labelled antigen were sorted from the skin draining LN using an Aurora CS for bulk RNAseq.