Transcription profiling of mouse soleus muscle to detect differently expressed genes between male BERKO and WT mice

Male skeletal muscles are generally faster and have a higher maximum power output than female muscles. Conversely, during repeated contractions female muscles sre generally more fatigue resistant and recover faster. we hypothesized that estrogen receptor beta is involved in this gender difference. The Affymetrix micorarray were used to deteced the differently expressed genes between male BERKO and WT mice in soleus muscles. Soleus muscles from 3 individual mouse of each genotype were used to perform the experiment.相较于雌性骨骼肌，雄性骨骼肌通常收缩速度更快，最大输出功率更高。与之相反，在反复收缩过程中，雌性骨骼肌往往具备更强的抗疲劳能力且恢复速度更快。本研究推测，雌激素受体β（estrogen receptor beta）参与了这一性别差异的形成。本研究使用Affymetrix基因芯片（Affymetrix microarray）检测了雄性BERKO小鼠与野生型（WT）小鼠比目鱼肌中的差异表达基因。本实验每组基因型均选取3只独立个体的比目鱼肌完成。