Temporal control of species-specific motor neuron differentiation [Mm]

How species-specific developmental timing is controlled is largely unknown. By following human embryonic stem cell (hESC) and mouse stem cell (mESC) differentiation to motor neurons through detailed RNA-sequencing time courses, we wish to find if there is a global scaling factor between species RNA samples from mouse and human in vitro differentiations. Human and mouse stem cells monolayer differentiations towards motor neurons upon addition of Retinoic Acid and a Sonic Hedgehog agonist. Mouse samples were collected every 12h for the first three days and daily up to day 7 in triplicates. Human samples are collected daily in triplicates up to day 15.