Gene expression profile at single cell level of leg muscles from E18.5-day WT and SYISL KO fetal mice from the same litter

To further explore the mechanism of SYISL knockout on muscle fiber development, we performed 10X Genomics single-cell RNA sequencing of leg muscles from E18.5-day WT and KO fetal mice from the same litter. Overall design: Samples were collected from the leg muscles of E18.5 embryos from both genotypes, with each condition including two biological replicates. Analysis was conducted using single-cell RNA sequencing (scRNA-seq).