Microarray analysis reveals changes in tRNA-derived small RNAs (tsRNAs) expression in mice with septic cardiomyopathy

Our previous work has focused on the relationship between non-coding RNAs and sepsis, so we chose to use microarray analysis to investigate the relationship between septic cardiomyopathy and a novel class of small non-coding RNA - tRNA-derived small RNAs(tsRNAs). Meanwhile, tRNA-derived small RNA has been studied in many cardiovascular diseases, but the relationship between tsRNAs and septic cardiomyopathy has not been investigated. We sought to analyze changes of tsRNAs expression profile in septic cardiomyopathy and reveal an important role for tsRNAs. C57BL6 male wild type (WT) mice (each 9 weeks old and weighing 20-25g) were purchased from the Department of Central South University Laboratory Zoology (Changsha, China). The mice were reared with strict specific-pathogen-free (SPF) conditions (room temperature at 25℃ and 12 h light-dark cycle), and had free access to standard rodent water and food. After one week of acclimatization, the mice were randomly separated into two groups: Control and LPS24h groups. Intraperitoneal injection of LPS (10mg/kg) was used to establish the model of septic cardiomyopathy. After 24 hours, the heart and serum were collected after sacrificed by cervical dislocation. Three mice in LPS24h Group and sham-operation group were used in the follow-up microarray experiment.