Impact of depletion of groucho or notch on transcription in Drosophila intestinal stem cells (ISCs).

Purpose: To analysis impacts of groucho and notch on transcription in Drosophila ISCs by mRNA-seq. Methods: mRNA-seq of RNA extracted from FACS sorted Drosophila ISCs. Results: Groucho loss in ISCs leads to disruption of lateral inhibition,and knocking down notch in ISCs down-regulates expression of e(spl) factors. Isolation and amplification of total mRNA of FACS sorted ISCs from Dl>UAS-GFP(Ctrl) and Dl>UAS-GFP, groucho-RNAi or Dl>UAS-GFP, notch-RNAi guts, followed by deep sequencing analysis. Arcturus PicoPure RNA isolation kit (Applied Biosystems) and Arcturus RiboAmp HS PLUS RNA amplification kit were used for RNA extraction and amplification separately.