Genome-wide maping of chromatin state and gene exoression in hematopoietic stem and progenitor cells.

We report the application of H3K27me3 ChI-seq assays in hematopoietic stem and progenitors cells (HSPCs) from p53+/+ and p53R248W/+ mutant mice. We also performed RNA-seq assays to examine the impact of p53 on gene expression in hematopoietic stem and progenitor cells. Overall design: Examination of histone modification H3K27me3 in p53+/+ and p53R248W/+ HSPCs (Lin-Kit+). Examine gene expression in p53+/+, p53R248W/+, and p53-/- HSPCs (LSKs)