Transcriptome comparison of the wild type strain Azoarcus sp. BH72 with that of nifLA knockout strain under nitrogen fixing condition. Azoarcus sp.

Azoarcus sp. BH72 is known to express nitrogenase genes endophytically in rice seedlings in gnotobiotic culture. Availability of fixed nitrogen is one of the important signals regulating the transcription of nitrogenase genes and hence nitrogen fixing activity. NifA is the essential transcription activator of nif genes. RNA isolated from the nifA knockout mutant of strain BH72 was compared with the transcriptome of wild type under nitrogen fixing condition using a global genome wide microarray approach and the differences in the gene expression profile were monitered. Overall design: RNA isolated from wild type strain BH72 and nifLA mutant strain BHLAO grown respectively under microaerobic nitrogen fixing condition with glutamate as poor nitrogen source was used for two color whole genome microarray approach