mRNA transcriptome sequencing of heart tissues from 5-month-old wildtype mice, 21-month-old wildtype mice, and 21-month-old wildtype mice treated with Ixekizumab

We used mRNA amplification transcriptome sequencing to analyze the expression changes of different genes in the heart tissue of 5-month-old wildtype mice, 21-month-old wildtype mice, and 21-month-old wildtype mice treated with Ixekizumab. Differentially expressed genes (DEGs) were screened by "FindAllMarkers" analysis (| log2FC |>1, p value<0.05). Use the "ClusterProfiler" to perform GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis on differential genes, and visualize the KEGG and GO pathway enrichment analysis results using the "enrichplot" package.