Heterosis research: parental lines - WGS

Heterosis, the superior performance of hybrids over their parents, has been exploited systematically in crop breeding and is considered a crucial asset for addressing global food demands. To investigate the genetic basis of midparent heterosis in hybrid populations derived from diverse parental lines, a large hybrid dataset comprising 5,234 single crosses produced from 588 parental lines were used to mapping the heterotic QTL. The raw sequenced data of 131 parental lines has previously released at the European Nucleotide Archive (https://www.ebi.ac.uk/ena/) under the following Project IDs PRJEB48738. The newly 452 wheat samples were whole genome shotgun paired-end sequenced (WGS) using a NovaSeq 6000 system and an average genome coverage of 3-fold. Libraries were prepared using the Nextera DNA Flex Library Prep according to Illumina protocols and pooled afterwards in an equimolar manner. Passport data for these samples were collected by Guoliang et al. 2025.