Text S1 - Nematode-Induced Interference with Vaccination Efficacy Targets Follicular T Helper Cell Induction and Is Preserved after Termination of Infection

Figure S1. L. sigmodontis infection reduces quantity but not quality of DNP-specific IgG. Six- to eight-week-old BALB/c mice were naturally infected with L. sigmodontis (blue squares, n = 10) or left non-infected (white squares, n = 10) and vaccinated with 100 µg DNP-KLH/Alum i.p. at day 60 p.i. Control mice (black circles, n = 2) were infected but not vaccinated. DNP-specific IgG1, IgG2a and IgG2b was quantified in sera of mice. Results are expressed as mean ± SEM of pooled data derived from two independent experiments. Asterisks indicate significant differences of the mean of DNP38-specific Ig in non-infected and infected mice (Two-way ANOVA). Figure S2. L. sigmodontis infection suppresses humoral response to TD vaccination in the presence and absence of MF. Quantification of DNP-specific IgG1, IgG2a and IgG2b in sera of DNP-KLH-vaccinated non-infected (white squares, n = 12) and L. sigmodontis-infected (A) microfilaraemic mice (blue squares, n = 7) and (B) non-microfilaraemic mice (blue squares, n = 12). Results are expressed as mean ± SEM of pooled data derived from four independent experiments. Asterisks indicate significant differences of the mean of DNP-specific Ig in non-infected and infected mice (Two-way ANOVA). Figure S3. L. sigmodontis- specific Ig response during infection. Six- to eight-week-old BALB/c mice were naturally infected with L. sigmodontis and not vaccinated (black circles). Non-infected mice (open squares) and infected mice (grey squares) were vaccinated with 100 µg DNP-KLH/Alum i.p. L. sigmodontis-specific Ig in the sera was quantified by ELISA at the indicated time points post infection. Results are expressed as mean ± SEM of pooled data derived from at least five independent experiments (days 14 and 102: n = 4, days 30, 60 and 81: n = 6).
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