Characterization of Human Skeletal Muscle Biopsy Samples Using Shotgun Proteomics
收藏NIAID Data Ecosystem2026-03-06 收录
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We characterized the human muscle proteome by studying muscle biopsy specimens through four different workflows, using 1 or 2D peptide separation, SDS gels, or differential solubilization. By performing MS/MS analyses of 178 4-h LC separations derived from 31 patients, we identified more than 2000 proteins, and determined how 370 very abundant proteins behave upon differential solubilization. The resulting semiquantitative database should serve as a resource for muscle biochemistry.
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2016-02-26
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