Next generation sequencing facilitates quantitative analysis of control samples and nervous necrosis (VNN) virus infection samples orange-spotted grouper transcriptomes

Purpose:To study the molecular mechanism of nerve necrosis (VNN) virus infection, and to provide help for molecular breeding and further development of Marine fishery Results: we found that 17,939 genes are elevated after NNV infection compared with control group, suggesting that NNV infection can strongly induce the expression of immune associated genes. In summary, we obtained high quality transcriptome of orange-spotted grouper and comparative analysis of transcriptome analysis reveals dramatically perturbation of gene expression with NNV infection. mRNA profiles of 20-day old control and nervous necrosis (VNN) virus infection orange-spotted groupers (Epinephelus coioides) were generated by deep sequencing, in triplicate, using HiSeq 4000.