Diversity and evolution of chitin synthases in oomycetes
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Supporting data for our paper on "Diversity and evolution of chitin synthases in oomycetes". File S1 contains the full-length CHS sequences separated into clades (P1 and 2, S1-6, E. dicksonii, and 3 outgroups). S2 is like S1, but CHS motifs a-h only. Files S3a and S3b contains the sequences spanning the MIT domain. S3b contains an alternative version where the Pfam-identified domains where picked out from sequences alignment by MUSCLE. Files S4a-S4c contains the multiple sequence alignments for full-length CHSs from Clustal Omega v1.2.4, MAFFT v7.215, and MUSCLE v3.8.31. Files S5a-S5c contains the multiple sequence alignments for the MIT domain from Clustal Omega v1.2.4, MAFFT v7.215, and MUSCLE v3.8.31. Files S6a-S6c contains the multiple sequence alignments for the MIT domain from Clustal Omega v1.2.4, MAFFT v7.215, and MUSCLE v3.8.31. S7 contains the multiple sequence alignment used to generate supplementary fig. S5. S8a and S8b contains the tree in Newick format that where used to produced Suppl Figure S7. Files S9a-S9c contains the multiple sequence alignments from Clustal Omega v1.2.4, MAFFT v7.215, and MUSCLE v3.8.31 for the MIT domain and its upstream region of the Pfam seed. NOTE: these multiple sequence alignments are in a different format than in the other supplementary files due to long sequence identifiers
本数据集为论文《卵菌几丁质合酶(CHS)的多样性与演化》的配套支撑数据。文件S1包含按演化支(P1、P2、S1-6、E. dicksonii以及3个外类群)划分的全长几丁质合酶序列。文件S2与S1格式类似,但仅包含几丁质合酶基序a至h。文件S3a与S3b包含覆盖MIT结构域(MIT domain)的序列;其中S3b包含一个备选版本,其Pfam鉴定的结构域通过MUSCLE工具从序列比对中提取得到。文件S4a至S4c包含分别由Clustal Omega v1.2.4、MAFFT v7.215以及MUSCLE v3.8.31生成的全长几丁质合酶多序列比对结果。文件S5a至S5c包含分别由Clustal Omega v1.2.4、MAFFT v7.215以及MUSCLE v3.8.31生成的MIT结构域多序列比对结果。文件S6a至S6c包含分别由Clustal Omega v1.2.4、MAFFT v7.215以及MUSCLE v3.8.31生成的MIT结构域多序列比对结果。文件S7包含用于生成补充图S5的多序列比对结果。文件S8a与S8b包含用于生成补充图S7的Newick格式系统发育树文件。文件S9a至S9c包含分别由Clustal Omega v1.2.4、MAFFT v7.215以及MUSCLE v3.8.31生成的、针对Pfam种子序列的MIT结构域及其上游区域的多序列比对结果。注意:由于序列标识符过长,本部分的多序列比对文件格式与其他补充文件存在差异。




