Gene expression signatures of melatonin-treated dental papilla cells during odontoblastic differentiation

To further reveal the regulatory mechanism of melatoin-mediated odontoblastic differentiation during tooth development, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to modulate melatonin-induced odontoblastic differentiation. Rat dental papilla cells were primary cultured from PN1-3 tooth germ tissue, and treated with melatonin at the concentration of 10-8M for 3 days along with odontoblastic differentiation induction medium (OS). Current microarray analysis generally profiled a total of 181 upregulated genes and 580 downregulated genes between two groups.为进一步阐明牙发育过程中褪黑素（melatonin）介导的成牙本质细胞分化调控机制，本研究以全基因组微阵列表达谱作为筛选平台，旨在筛选可调控褪黑素诱导的成牙本质细胞分化的潜在功能基因。本研究从出生后1-3天（PN1-3）的大鼠牙胚组织中原代培养大鼠牙乳头细胞，以浓度为10^-8 M的褪黑素联合成牙本质细胞分化诱导培养基（OS）处理细胞3天。本次微阵列分析共鉴定得到两组样本间的181个上调基因与580个下调基因。