The effects of SCAP deletion on gene expression of activated B cells. The effects of SCAP deletion on gene expression of activated B cells

To investigate the role of SREBP signaling in B cell activation, we stimulated SCAP+/+CD19Cre/+ (WT) and SCAPfl/fl CD19Cre/+ (KO) B cells with LPS, anti-CD40 or anti-IgM for 24 hrs and performed RNA sequencing to identifiy the differences. Overall design: We performed RNA seqencing comparing WT and KO B cell unstimulated (time 0) or stimualted with LPS, anti-CD40 or anti-IgM for 24 hrs